دعوه لحضور محاضرة
يسرنا دعوتكم لحضور المحاضرة التي سيلقيها الدكتور الباحث أحمد طلفاح
من معهد لايبنتز للعلوم التحليلية (ISAS) في دورتموند، المانيا
Leibniz Institut für Analytische Wissenschaften (ISAS)
وذلك الساعة الواحدة ظهرا يوم الثلاثاء الموافق 23/12/2014
مركز حمدي منكو للبحوث العلمية / مدرج علي حمدي منكو
Online Nuclear Magnetic Resonance (NMR) online detection of living cancer cells and micro magnetic imaging resonance
Abstract: Nuclear Magnetic Resonance (NMR) is a powerful analytical technique with a wide range of application field, and the NMR spectroscopy is a very information-rich and reliable analytical method which gives comprehensive chemical information about the composition of unknown materials. Typical NMR analyses are carried out in 5 and 3 mm sample tubes with approximately (700, 200) μL sample, Measuring small and ultra-smaller samples volumes are not possible due to the limit of detection (LOD).
A Microstrip NMR detector was designed to enable investigations of volume-limited samples in the low nanoliter range. Therefore NMR spectra of sample quantities in the 100 pmol range can be obtained with this microprobe in a few seconds. Moreover, the planar geometry of the detector is suitable to the size and geometry requirements such different kind of microfluidic cellular sample holders.
Since NMR is a nondestructive technique and high speciation performance, it is one of the major technologies for metabolic profiling, hence allows in vivo measurements of biological cells.
In the ongoing research, we focus on metabolic analysis of living human cancer cells; the obtained data about the concentrations and production \ degradation rates of the metabolites give direct evidence of the present status of the cell, which assist us to understand biochemical pathways. A cellular microfluidic NMR sample holder was designed with temperature and proper gas atmosphere controlled environment in order to maintain the viability of the biological cells at near physiological conditions for the long-term in vitro studies.
As a near future investigation, cancer living cells samples will be treated with metal based anti-cancer drugs (e.g. platinum based drugs); the metabolomics respond analyses combined with the (1H,195Pt) NMR studies (signals and correlation times) will be employed to understand the molecular mechanism of anticancer drugs action, and to find out the transport mechanism through the cellular membrane, by quantitative calculation of the organic and nonorganic Platinum NMR signal of the treated cells, the pseudo-first-order rate constant for the reaction of anti-cancer drug with water will be defined, which is indeed a very important factor to monitor the efficiency, aging and degradation of metal-based anti-cancer drugs.