Flow cytometry is a technology that measures and analyzes multiple physical characteristics of single cells as they flow in a fluid stream through a beam of light.Flow cytometry can identify and isolate the target cell population. This process enables to verify the purity of the samples by detecting and removing unwanted cells or debris. BDFACS CANTO II flow cytometer has three lasers: Blue (488mn), Red (633nm) and Violet (405nm). It can detect 10 parameters (8 colours plus forward scatter and side scatter). Its suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.  It supports a broad range of research applications includes; immunophenotyping, cell sorting, cell cycle analysis, cell viability apoptosis, cancer diagnosis and drug discovery and development. these data are acquired through BD FACSDIVAtm digital software